Precise Gridding of Microarray Images by Detecting and Correcting Rotations in Subarrays
نویسندگان
چکیده
DNA microarrays are an important tool for massive gene expression profiling and genotyping studies. A microarray consists of thousands of spots that must be scanned and digitized for further analysis. However, the mechanical constraints and other printing issues increase the difficulty in automatic analysis, most of the applications released up to now need different levels of human intervention, which inevitably reduce the efficiency and reproducibility of the entire process. In this paper, we present a precise microarray gridding method, in which potential problems in grid alignment and possible rotation in subarrays are detected and corrected. The gridding process is fully automatic, which eliminates the need for human intervention.
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